An HPLC–MS/MS method for the separation of α-retinyl esters from retinyl esters
نویسندگان
چکیده
منابع مشابه
Retinol and retinyl esters: biochemistry and physiology.
By definition, a vitamin is a substance that must be obtained regularly from the diet. Vitamin A must be acquired from the diet, but unlike most vitamins, it can also be stored within the body in relatively high levels. For humans living in developed nations or animals living in present-day vivariums, stored vitamin A concentrations can become relatively high, reaching levels that can protect a...
متن کاملThe fate of intracardially administered retinyl esters in the rat.
Irrespective of the form of vitamin A fed to rats, it appears in blood almost exclusively as retinyl palmitate, and the liver always stores only this ester. Differences in the fatty acid composition of the dietary fats do not seem to have any influence on the type of retinyl ester absorbed and stored by the rat (Mahadevan & Ganguly, 1961; Mahadevan, Seshadri Sastry & Ganguly, 1963). It was ther...
متن کاملRetinyl esters are hydrolyzed in early endosomes of J774 macrophages.
The aim of the current study was to identify the subcellular compartment(s) responsible for the hydrolysis of chylomicron remnant-retinyl esters, in J774.1 cells. The cells were incubated with medium containing chylomicron remnant [(3)H]retinyl ester. Subcellular fractionation was used to separate early endosomes from late endosomes and lysosomes. About 26% and 80% of the total [(3)H]retinyl es...
متن کاملFluorometric determination of total retinyl esters in triglyceride-rich lipoproteins.
A time-consuming sample preparation and measuring procedure is required for the quantitation of retinyl palmitate by HPLC. We developed a fluorometric method for the determination of total retinyl esters in chylomicrons, chylomicron remnants, and VLDL. This method is precise, sensitive, rapid, simple, and particularly useful for large-scale studies of postprandial lipid metabolism. Because the ...
متن کاملRetinol and retinyl esters in parenchymal and nonparenchymal
Chronic ethanol consumption reduces the liver retinoid store in man and rat. We have studied the effect of ethanol on some aspects of retinoid metabolism in parenchymal and nonparenchymal liver cells. Rats fed 36% of total energy intake as ethanol for 5-6 weeks had the liver retinoid concentration reduced to about one-third, as compared to pair-fed controls. The reduction in liver retinoid affe...
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ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2016
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2016.06.043